A multilineage analysis of EGFP expression in blood and bone marrow cells was obtained 13 weeks after transplantation into irradiated Ly5.2+ recipients. As shown in Table 1 and Figure 3a, the group of mice having received cells transduced with the higher MOI showed a significantly increased frequency of EGFP+ cells in all developmental lineages and stages of the PB and BM analyzed (eg six fold increase in Ly5.1+ leukocytes and Sca1+ progenitor cells). CD11b+ myeloid cells expressing EGFP also showed a three fold brighter fluorescence when compared with those from the low MOI group (P = 0.025).
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